Kinetics of Glycerol Acclimated Biomass: Implications on Plant Operations and Performance


  • Sarah Dailey and Paul Pitt - Hazen and Sawyer
  • Robert Sharp and Andrew Brace - Manhattan College
  • Allen Deur and Keith Beckmann - NYCDEP

Glycerol-based carbon sources have gained significant attention from the wastewater industry as an alternative supplemental carbon source for denitrification because they are a waste product of biodiesel production, do not pose health and safety risks like methanol and ethanol, do not have kinetic limitations in cold weather, and have proven to be effective at pilot and full-scale. In this paper, we present results from a full-scale demonstration of an AT-3 Separate Centrate Treatment process with glycerol as the added carbon source. In this study, we observed that that glycerol acclimated biomass (GAB) preferentially performed denitratation (NO3 to NO2) at a very high rate, which resulted in nitrite accumulation. The denitritation (NO2 to N2) was also found to be highly dependent on the presence of residual nitrate. Batch tests experiments were carried out to better understand these kinetic phenomena and to investigate potential operational changes that could be made to reduce the impacts of the nitrite accumulation during glycerol-based denitrification.

In this paper and presentation, we will present the full complement of data characterizing the results from the kinetic and microbiological testing, along with a discussion of possible implications of GAB on operations and performance of both main plant BNR and SCT.

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Horizons Fall 2017 (pdf)

Horizons showcases significant water, wastewater, reuse, and stormwater projects and innovations that help our clients to achieve their goals, and can help you achieve yours. Articles are written by top engineers and process group leaders, demonstrating and explaining the beneficial application of a variety of technologies and tools.

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